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KMID : 0880520100460030163
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Chonnam Medical Journal
2010 Volume.46 No. 3 p.163 ~ p.169
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Altered Renal Expression of Acid-base Transporters in Rats with Glycerol-induced Tubular Injury
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Ma Seong-Kwon
Bae Eun-Hui
Lee Jong-Un
Kim Soo-Wan
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Abstract
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The present study aimed to investigate the altered regulation of renal tubular acid-base transporters in rats with glycerol-induced tubular injury. Male Sprague-Dawley rats were used. Rats were injected with 50% glycerol in normal saline (7 mL/kg, i.m.) after water deprivation for 12 hours and were then sacrificed at 24 hours after the glycerol injection. The expression of Na,K-ATPase α1-subunit, type 3 Na+/H+ exchanger (NHE3), type 1 Na+:HCO3- (NBC1), and B1-subunit of apical H+-ATPase was determined in the kidney by semiquantitative immunoblotting and immunohistochemistry. In the experimental rats, creatinine clearance was decreased, whereas fractional sodium excretion was not changed. Urine pH and bicarbonate concentrations were decreased, although plasma pH and bicarbonate concentrations were not changed. In the experimental group, the protein expression of Na,K-ATPase α1-subunit was decreased in the cortex and outer stripe of outer medulla (cortex/OSOM) and inner stripe of outer medulla (ISOM) but increased in the inner medulla. The expression of NHE3 was decreased in the cortex/OSOM and ISOM, and that of NBC1 was decreased in the cortex/OSOM. By contrast, the expression of H+-ATPase was increased in the cortex/OSOM and inner medulla but was unchanged in the ISOM. Immunohistochemical analyses confirmed the immunoblotting data. In glycerol-induced tubular injury, the down-regulation of Na,K-ATPase α1-subunit, NHE3, and NBC1 may contribute to impaired tubular reabsorption of sodium and bicarbonate. The upregulation of H+-ATPase may play a preventive role against the development of metabolic acidosis.
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KEYWORD
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Sodium-hydrogen exchanger 3, Sodium, Bicarbonates, Membrain transport proteins
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